Journal: Investigative Ophthalmology & Visual Science
Article Title: Apolipoprotein A1 Modulates Teff/Treg Balance Through Scavenger Receptor Class B Type I-Dependent Mechanisms in Experimental Autoimmune Uveitis
doi: 10.1167/iovs.63.8.23
Figure Lengend Snippet: APOA1 modulates proliferation and differentiation of T cells. (A, B) The viability of T cells was affected by APOA1 inconspicuously at 5 µM and mildly at 10 µM, but significantly at 20 µM and 40 µM (n = 5). (C, D) The proliferation of CD3 + T cells was markedly inhibited by APOA1 at concentrations of 5 µM and 10 µM (n = 5). (E, F) The induction of Th1 differentiation (CD4 + IFN-γ + ) was reduced by APOA1 (n = 5). (G, H) The induction of Treg differentiation (CD4 + CD25 + Foxp3 + ) was expedited by APOA1 (n = 4). The values represent the mean ± SD. nsp>0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Article Snippet: The cultures were also supplemented with Th1 or Treg induction condition (ImmunoCult mouse Th1 or Treg differentiation supplement, Stemcell Technologies) for respective conversion according to the manufacturer's instructions.
Techniques: